Neuronal tracing with DiI: decalcification, cryosectioning, and photoconversion for light and electron microscopic analysis.
نویسندگان
چکیده
منابع مشابه
Neuronal tracing with DiI: decalcification, cryosectioning, and photoconversion for light and electron microscopic analysis.
The molecule 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) is a fluorescent dye which diffuses within cell membranes. The properties of DiI diffusion and fluorescence are maintained in aldehyde-fixed tissue, thereby allowing selective neuronal tracing post mortem. We describe three modifications of this tracing method. First, while DiL diffuses along neuronal membrane...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1990
ISSN: 0022-1554,1551-5044
DOI: 10.1177/38.5.2185313